Alain Brans, Fabrice Bouillenne, Iris Tham, Anne-Marie Matton, Moreno Galleni, André Matagne

Protein Factory - ULiège
" At Protein Factory, the protein expression and purification facility of the Centre for Protein Engineering (CIP; ULiège), we have produced proteins for COVID-19 diagnostics. Thus, in a collaborative effort with the GIGA Institute at ULiège (Haruko Takeda, in particular), we have been involved in the implementation of alternative rapid and reliable tests, based on loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) assays, which both appear as promising alternative to the standard quantitative reverse transcription (qRT) PCR procedure. In addition, for the sake of increased reliability, the CRISPR-Cas12 / Cas13 reporter detection systems have also been implemented. In order not to depend on commercial products, we produced at Protein Factory all the enzymes necessary to perform these tests internally, regardless of any supply shortage or price inflation. Thus, the following enzymes are now available at the CIP for further development: Cas12a (DNA cleavage) and Cas13a (RNA cleavage) for CRISPR/Cas assays, Rtx exo- (Reverse transcriptase xenopolymerase) for thermostable DNA synthesis, DNA Polymerase PolA (Large Fragment) from Bacillus stearothermophilus for LAMP and DNA Polymerase PolA (Large Fragment) from Bacillus subtilis 168, UvsX, UvsY, and GP32 from T4 phage, all four enzymes for RPA. "
Contact: Alain Brans This email address is being protected from spambots. You need JavaScript enabled to view it.

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